Black hole quenchers (BHQ) and 3' blocking in Real-time PCR
(by blackhole from abuse.plus.com)
Fri Jan 16 10:05:24 EST 2009
Historians believe that in newspost
<a0a35eb6-50d6-48bb-8eac-d98660c335f6 from n10g2000vbl.googlegroups.com> on
Thu, 15 Jan 2009, chovek69 <ivanoov from gmail.com> penned the following
>The company states exclusively that they can do 3' - BHQ labeling but
>is this also mean that the 3'-OH will be blocked by the quencher
>against extension or I must order 3' phosphorylation in addition.
No need for 3' phosphorylation. They will use a column that has quencher
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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