Black hole quenchers (BHQ) and 3' blocking in Real-time PCR

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Sat Jan 17 09:08:13 EST 2009


On Jan 16, 5:05 pm, Duncan Clark <blackh... from abuse.plus.com> wrote:
> Historians believe that in newspost
> <a0a35eb6-50d6-48bb-8eac-d98660c33... from n10g2000vbl.googlegroups.com> on
> Thu, 15 Jan 2009, chovek69 <ivan... from gmail.com> penned the following
> literary masterpiece:
>
> >The company states exclusively that they can do 3' - BHQ labeling but
> >is this also mean that the 3'-OH will be blocked by the quencher
> >against extension  or I must order 3' phosphorylation in addition.
>
> No need for 3' phosphorylation. They will use a column that has quencher
> attached.
>
> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.

Thanks alot Duncan .... your suggestions are always the best


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