(pas de sujet)

shifali chatrath via methods%40net.bio.net (by shifalich from rediffmail.com)
Fri Jan 23 05:02:48 EST 2009


  
We regularly purify proteins on RPHPLC C18 column. I have never heard of any protein getting pptd. with ACN. I suggest you to go ahed with your purification!
All the best!

On Thu, 22 Jan 2009 Mouldi SAIDI wrote :
>Dear all,
>I would like to ask if the separation of casein using HPLC, C18 RP and acetonitrile/water TFA  is possible.
>I'm afraid that acetonitrile precipitates proteins?
>Best regards
>
>_______________________________________________
>Methods mailing list
>Methods from net.bio.net
>http://www.bio.net/biomail/listinfo/methods


Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore
Singapore
+65-65161210
+65-96393449


More information about the Methods mailing list