(pas de sujet)

shifali chatrath via methods%40net.bio.net (by shifalich from rediffmail.com)
Fri Jan 23 05:02:48 EST 2009

We regularly purify proteins on RPHPLC C18 column. I have never heard of any protein getting pptd. with ACN. I suggest you to go ahed with your purification!
All the best!

On Thu, 22 Jan 2009 Mouldi SAIDI wrote :
>Dear all,
>I would like to ask if the separation of casein using HPLC, C18 RP and acetonitrile/water TFA  is possible.
>I'm afraid that acetonitrile precipitates proteins?
>Best regards
>Methods mailing list
>Methods from net.bio.net

Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore

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