(pas de sujet)
(by shifalich from rediffmail.com)
Fri Jan 23 05:02:48 EST 2009
We regularly purify proteins on RPHPLC C18 column. I have never heard of any protein getting pptd. with ACN. I suggest you to go ahed with your purification!
All the best!
On Thu, 22 Jan 2009 Mouldi SAIDI wrote :
>I would like to ask if the separation of casein using HPLC, C18 RP and acetonitrile/water TFA is possible.
>I'm afraid that acetonitrile precipitates proteins?
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