Isolation of cell membrane protein

[Han]

neda akbari <depbioakbari from yahoo.com> wrote in
news:mailman.1614.1233164292.29717.methods from net.bio.net: 

> i want to isolate an enzyme which bound to cell membrane. i used
> Tris-buffer with triton X-100 ( 0.5 %) pH= 8 by sonication but most of
> them didnt isolated by comparing with SDS-PAGE.can you help me? thank
> you in advance 
> 
It's possible the enzyme isn't soluble in Triton, or that it requires more 
than 0.5%.  Many membrane proteins are insoluble in Triton.  One of the 
ways to prepare cytoskeletal proteins is to remove the Titon-soluble 
proteins, and then study what's left.

-- 
Best regards
Han 
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