strand displacement activity assay

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Wed Jul 8 08:21:19 EST 2009


Hello fellow scientists,
I am trying to come up with an assay to determine strand displacement
activity of T4 DNA pol. Any ideas? In general it is accepted that T4
pol has very little strand displacement, but for my application I need
complete lack of such (single molecule assay). Thanks in advance old
timers....


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