strand displacement activity assay

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Wed Jul 8 23:29:40 EST 2009


In <ad35d21c-323b-46c2-9668-fbc669425604 from t21g2000yqi.googlegroups.com>,
 peter <peter.ianakiev from gmail.com> wrote:

> Thanks for the suggestion,
> Unfortunately I don't have permit for radioactivity in this lab....I
> was thinking something along annealing oligo that have a point
> mutation that would abolish restriction site and then making dsDNA
> with another oligo . If it gets displaced it will be cut with the RE,
> if it does not get cut - oligo was not displaced. That is assuming
> 100% annealing though ....

If you have access to a UV-light box, you can FITC label the 3' end.
This works, although the sensitivity is lower than 32P.  It is more
suitable for high-thruput applications.

A

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