Biotin contamination of BSA

ruchi jain via methods%40net.bio.net (by ruchijainjnu from gmail.com)
Thu Jul 16 08:01:43 EST 2009


Do the blocking with either gelatin or skimmed milk. We have been using
skimmed milk and I would say its a cheap and a very good way of blocking in
westerns.
Best
Ruchi

On Thu, Jul 16, 2009 at 3:24 PM, WS <novalidaddress from nurfuerspam.de> wrote:

> Dear Experts,
>
> I noticed that "ordinary" BSA is unsuitable for blocking westerns when
> streptavidin HRP is used, as it causes high background by its biotin
> content. Where does the Biotin contamination come from and how may I
> get rid of it (except buying biotin free BSA)? Is it covalently
> linked?
>
> Best regards,
>
> Wo
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-- 
Ruchi Jain, Ph.D.
Postdoc Associate
Internal Medicine-Infectious Diseases
Yale University
ruchi.jain from yale.edu
203-737-1197


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