flurescent proteins

[Dr Engelbert Buxbaum]

Am 14.07.2009, 07:19 Uhr, schrieb Azam Rahimpour <rahimpour_a from yahoo.com>:

> hi
> I have a question about measuring fluorescence intensity of gfp, is it  
> important to use the exact excitation emission wavelenght (488/509) or  
> applying wavelenghts neat to that might works? does anyone can help?

Peaks in optical spectroscopy are usually quite wide, so in principle you  
can use wavelengths "near" the maximum too. Obviously, the further you go  
away from the maximum, the lower the fluorescence observed.

For a review on GFP see

@article{Tsi-98,
         AUTHOR= {Tsien, Roger Y.},
         TITLE= {The green fluorescent protein},
         JOURNAL= {Annual Review of Biochemistry},
         VOLUME= {67},
         PAGES= {509-544},
         YEAR= {1998},
         DOI= {10.1146/annurev.biochem.67.1.509}
}