cleaning coverslip for protein/cell attachment

[Dr Engelbert Buxbaum]

Am 15.07.2009, 21:11 Uhr, schrieb guanyuan fu <guanyuanfu from gmail.com>:

> Hi, all:
>
> I have some trouble with cleansing my coverslips for protein coating. The
> coverslips (22*22 mm) seemed to be very hydrophobic,
> The procedure is listed below:
>
> 1) Wash the coverslips with chromic sulfuric acid (fisherbrand cleaning
> solution) for overnight.
> 2) Rinse with running tape water for 3 hrs to remove chemical residues  
> from
> step 1.
> 3) Rinse with distilled water 3 times each 30 mins.
> 4) Wash coverslips with 100% alcohol (mixed alcohol, not pure ethanol)  
> for 2
> hrs
> 5) Discard alcohol, and separate coverslips one by one to let it air dry.
> 6) After dry, store in glass plate then autoclave.

chromium sulfuric acid you can make yourself by dissolving 10% potassium  
dichromate in conc. sulfuric acid (with the obious precautions). It is  
bright orange, and turns green when completely used up. In between, it  
looks brownish. I use a small volume of the acid, and then discard it  
after one time use.

Place slips into the acid one by one, so that all surfaces are in contact  
with the chemical. Once they have been rinsed with water, place them into  
a holder that keeps them a mm apart, again to ensure that all surfaces are  
accessible to the solutions. Obviously, once slips come out of the acid,  
you can touch them only with tweezers, so that no new greese gets onto  
them.