protein extraction

riccardo senter via (by ricc_wparma from
Sun Jul 19 15:47:44 EST 2009

i'm a new entry.
i need to extract proteins from PBMC in order to perform a western blot and a functional assay of iNOS, which is a cytosolic homodimeric protein.
which is the best extraction protocol?

Another question: can i use PBMC pellets that have been storing for several years at -20°?


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