storing of enzyme

[Dr. Hiranya S. Roychowdhury]

Use a buffer (10mM Tris-EDTA, pH 6.5 to 7.2, or 50mM phosphate buffer 
should work) and add glycerol to 35% (as high as 50%).  Store in a -20C
freezer (no auto defrost).

Cellulase can also be stores freeze dried after the appropriate dialysis
against distilled water containing 1mM DTT.

>
> I have purified cellulase enzyme through His tage and interested to store
> the purified protein. I shall be glad to know the recepi for stable storge
> of purified enzyme.
>
>
>
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-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003