storing of enzyme

Dr. Hiranya S. Roychowdhury via methods%40net.bio.net (by hroychow from nmsu.edu)
Wed Jul 29 10:26:15 EST 2009


Mostly cellulose nitrate.  I had done it "way back when ..." using these.


> On Jul 28, 3:51 pm, "Dr. Hiranya S. Roychowdhury" <hroyc... from nmsu.edu>
> wrote:
>> Use a buffer (10mM Tris-EDTA, pH 6.5 to 7.2, or 50mM phosphate buffer
>> should work) and add glycerol to 35% (as high as 50%).  Store in a -20C
>> freezer (no auto defrost).
>>
>> Cellulase can also be stores freeze dried after the appropriate dialysis
>> against distilled water containing 1mM DTT.
>>
>
> Is dialysis tubing still made from cellulose?
>
> Nick
>
> --
> Nick Theodorakis
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-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003



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