Promoter amplification

Christian Praetorius via methods%40net.bio.net (by prae from gmx.net)
Mon Jun 8 04:24:10 EST 2009


Aawara Chowdhury <aawara from pontiff-playground.org> wrote:

>I don't know how long your piece is, but you could consider having
>it synthesized as a "mini-gene".

It's approximately 2,5kb, so my boss wouldn't be to glad about the
costs for ordering such a large piece of DNA. Since the BAC has some
appropriate restriction sites (I will only have to add them to the
plasmid, which is quite simple), I will try to go this route. And keep
the other one in my mind.

Christian

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