ER special dyes

[Cathal Garvey]

I have it on good authority from a labmate that sucrose-gradients are  
a great way to check for protein localisation. I gather it involves a  
long centrifuge cycle but yields very reliable results.

On 10 Jun 2009, at 11:06, Jinxiang Wang <jinxwang from scau.edu.cn> wrote:

> Dear Colleaguses,
>
> I am going to localize a protein which is from soybean.Bioinformatic
> analysis indicates it is maybe licalized in ER membrane.Initially I
> would likt to fuse my protein to GFP and check its
> localization.Alternatively I can employe antibody against my protein  
> to
> verify its localization. Certainly the later technique is
> time-consuming. Would you kindly give me your suggestions related to
> special marker dyes for ER or ER-special marker proteins? In addtion I
> know some special dyes and antibody for ER-special marker proteins are
> commercially availabe. I look foward to comments or ideas of experts  
> to
> guide me to select better dyes or marker protein Ab? Many thanks in  
> advance.
>
> Best regards.
>
> Jinxiang
>
>
> -- 
> ----------------------------------------------------
> Jinxiang Wang Ph.D
>
> College of Resources and Environment Science
> Root Biology Center
> South China Agricultural University
> Guangzhou 510642, P.R.China
>
> Email: jinxwang from scau.edu.cn
> Tel:   +86-20-85280156 (O)
> Fax:   +86-20-85281829
>
>
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods