Lectins

[hippie dream]

Hello All,

I recently purchased some TRITC conjugated Lectin from Triticum vulgaris
from Sigma (L5266-2MG). I intend to use this lectin to label EPS components
in a river biofilm. The lectin is shipped as a 	lyophilized powder. I
following the instruction of Neu & Lawrence (Methods in Microbiology, 2004).
They suggest preparing a stock solution by adding 1mL of buffer for every
1mg of lectin. 

My issue is that they suggest using either filter sterilized water or a
buffer. My quandary is that I don't know which to use. I don't even really
know how to assess which one to use and if I am using a buffer other than
water which do I use? If anyone has any ideas that would be fantastic.

Thanks!

Sam

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