Lectins

[Nick Theodorakis]

On Jun 23, 2:43 pm, hippie dream <tonightstheni... from gmail.com> wrote:
> Hello All,
>
> I recently purchased some TRITC conjugated Lectin from Triticum vulgaris
> from Sigma (L5266-2MG). I intend to use this lectin to label EPS components
> in a river biofilm. The lectin is shipped as a  lyophilized powder. I
> following the instruction of Neu & Lawrence (Methods in Microbiology, 2004).
> They suggest preparing a stock solution by adding 1mL of buffer for every
> 1mg of lectin.
>
> My issue is that they suggest using either filter sterilized water or a
> buffer. My quandary is that I don't know which to use. I don't even really
> know how to assess which one to use and if I am using a buffer other than
> water which do I use? If anyone has any ideas that would be fantastic.
>

Check the product sheet that came with the sample. If it was
lyophilized from buffer, then all you need to add is water because
buffer should be reconstituted along with the lectin.

Otherwise, choose conditions that are most compatibile with the
solubility of the lectin and won't interfere with downstream
applications.

Nick

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Nick Theodorakis
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