2D gel or MS? for comparison proteomic

[Dr Engelbert Buxbaum]

Am 26.02.2009, 08:56 Uhr, schrieb shifali  chatrath  
<shifalich from rediffmail.com>:


> we do all kinds of proteomic techniques. Also, a lot of mol. Bio. stuff.  
> and as far as I know, 2D and MS/MS can only be used you define the  
> composition of proteome in two types of cells (If you meant so....) but  
> not to quantitate.

Oh yes, 2D-electrophoresis can be used to compare levels of proteins in  
different samples, this is called "differential gel electrophoresis"  
(DIGE). From each sample you take an aliquote and label it with  
fluorescent dyes (or stable isotopes). An additional dye is used to label  
a mixture of all samples (internal standard). All samples are then mixed  
and run on the same gel. Then the fluorescence of a spot at different  
wavelengths is measured, the ratio is proportional to the ratio of  
proteins in the samples.