2D gel or MS? for comparison proteomic
(by shifalich from rediffmail.com)
Thu Mar 5 00:22:48 EST 2009
Yeah! I later checked with my colleagues.He also told me the same thing DIGE. But he also told its very cumbersome!
On Thu, 05 Mar 2009 Dr Engelbert Buxbaum wrote :
>Am 26.02.2009, 08:56 Uhr, schrieb shifali chatrath <shifalich from rediffmail.com>:
>>we do all kinds of proteomic techniques. Also, a lot of mol. Bio. stuff. and as far as I know, 2D and MS/MS can only be used you define the composition of proteome in two types of cells (If you meant so....) but not to quantitate.
>Oh yes, 2D-electrophoresis can be used to compare levels of proteins in different samples, this is called "differential gel electrophoresis" (DIGE). From each sample you take an aliquote and label it with fluorescent dyes (or stable isotopes). An additional dye is used to label a mixture of all samples (internal standard). All samples are then mixed and run on the same gel. Then the fluorescence of a spot at different wavelengths is measured, the ratio is proportional to the ratio of proteins in the samples.
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