Dopamine Measurment in PC12

[WS]

Hi Federico,

seems to me you get a precipitate that blocks the system.

you could

a) filter your sample through 0.2µM syringe filters
b) bring your sample to 10% MeOH and spin any precipitating material
down before injecting (better before step a) if you consider b)
c) use SPE columns for sample cleanup. Should work with methods for
catecholamine isolation from blood. You might refer to the clinical
chemistry department of your favorite hospital
d) use a small column of the same type as pre-column to protect the
actual HPLC column
e) reverse your column every 2nd or so run.

Best regards,

Wo