Peptide in solution
Paul J. Phelan
via methods%40net.bio.net
(by Paul.Phelan from tufts.edu)
Wed May 6 13:03:44 EST 2009
I would concentrate the peptide in a "Speed-vac": you freeze it and
then put it in a centrifuge under vacuum, the solvent will evaporate,
but the PBS salts will be concentrated along with the peptide.
Otherwise, you could lyophilize the peptide and re-dissolve it with
H2O, to whatever volume you like.
Quoting Yvonne Couch <yvonne.couch from pharm.ox.ac.uk>:
> I have a peptide dissolved in PBS at 1mg/ml, I want to concentrate it, is it
> possible to get the peptide out of solution in order to concentrate it?
>
> Cheers
>
> Yvonne
>
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