Primer extension kinetics
Wen Huang
via methods%40net.bio.net
(by huang_wen from ustc.edu)
Fri May 8 15:09:59 EST 2009
Hi
Does anybody know the kinetics of primer extension reaction? Is it
linear?
A related question is, under the temperature of a primer extension
reaction, does the primer-template hybrid gets disassociated? If they
do, to what extent, in other words, what magnitude is K as compared to
k in the primer extension step.
Thanks a lot.
Wen
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