Primer extension kinetics

Wen Huang via methods%40net.bio.net (by huang_wen from ustc.edu)
Fri May 8 15:09:59 EST 2009


Hi

Does anybody know the kinetics of primer extension reaction? Is it  
linear?

A related question is, under the temperature of a primer extension  
reaction, does the primer-template hybrid gets disassociated? If they  
do, to what extent, in other words, what magnitude is K as compared to  
k in the primer extension step.

Thanks a lot.

Wen



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