Seong Hwan Park
(by kuforen from gmail.com)
Mon May 11 20:02:29 EST 2009
The SMART oligo has three RNA bases at 3' end.
However, I've read an article about cDNA library technique, which
describes that authors used just DNA bases at 3' end of SMART oligo
and got a good result.
I think d(GGG) instead of r(GGG) will also work, but less efficiently.
In the patent document of SMART oligo, addition of phosphate to the 3'
end of r(GGG) made the background clean.
2009/5/12 Marc-Aurel Fuchs <m.fuchs from qub.ac.uk>:
> Can you replace the SMART oligo that comes with the Clontech RACE kit with a normal DNA primer of the same sequence?
> Or does it have to be an RNA oligo?
> Thanks, fuchse
> Methods mailing list
> Methods from net.bio.net
Seong Hwan Park, M.D., Ph.D.
Instructor in Legal Medicine,
College of Medicine, Korea University
126-1 Anadong 5ga, Seongbukgu, Seoul 136-704
E-mail: kuforen from gmail.com or pelvis from korea.ac.kr
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