pET23d vector

Ron Jenkins via methods%40net.bio.net (by jenkinsr from umich.edu)
Tue May 12 22:46:01 EST 2009


Aki,

Have you tried expressing it in TB media, and/or BL21 AI cells. 
Arabinose inducible tend to be less leaky which seems to help for 
insoluble proteins. Further, TB media does wonders. If this doesn't work 
check your pKa and check to the pH of your media. Buffered media (such 
as TB) which is richer tend to work better.

Ron



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