Methods Digest, Vol 48, Issue 10

TieQiao Wu via (by TieQiao.Wu from
Wed May 13 21:44:12 EST 2009


Does anyone have some experiences to purify insoluble GST-fusion
protein? I tried to purify GST-fusion protein recently, but it looks
that the protein did not bind to the beads, I did coommassie stain and
western blotting and results show the protein was expressed. I purified
the same protein last year although the fusion protein degraded
partially. In the cell suspension 1M DTT and 10% Sarkosyl were added
before sonication, 10% Triton X-100 was added after sonication, the
final concentrations are: 0.7% Sarkosyl, 2% Triton X-100 and 5mM DTT.

Thanks a lot!


This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 
If you have received this email in error please promptly delete this email and notify the system manager.

This email has been scanned by the MessageLabs Email Security System.
For more information please visit

More information about the Methods mailing list