Methods Digest, Vol 48, Issue 10

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Fri May 15 11:28:13 EST 2009


Am 13.05.2009, 22:44 Uhr, schrieb TieQiao Wu <TieQiao.Wu from bakeridi.edu.au>:

> Hi
>
> Does anyone have some experiences to purify insoluble GST-fusion
> protein? I tried to purify GST-fusion protein recently, but it looks
> that the protein did not bind to the beads, I did coommassie stain and
> western blotting and results show the protein was expressed.

That can happen, and you have two options:
- use a differnet tag (or a different position of tag, N- vs C-terminal)  
and hope that it will work
- do a conventional purification (IEC, SEC, HIC etc)


More information about the Methods mailing list