Monomeric Abeta detection in brain of APP tg mice using western blotting

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sat May 16 16:59:53 EST 2009


Hi Stanley,

for concentration, you might use a very low cut off mw dialysis
membrane and reduce the volume by "dialysis" against high mw poly-
ethyleneglycol.

You also first might might separate monomeric Abeta with 10kD cut off
spin column concentrator and the concentrate the flow through with a
1kd concentrator. In the latter step, you also may obtain some
desalting when you dilute your sample with dd water before the
concentration.

Just some thoughts and good luck!

Wo


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