Glyco stain

SIVAKUMAR via methods%40net.bio.net (by usiva from ufl.edu)
Wed May 20 16:59:28 EST 2009


Hi
Greetings
I have been working on archael protein. Does any one could help me 
to figure out the problem in glycosylation staining with Proq 
emerald 300. I am getting non specific staining that means it 
stains even nonglycosylated protein which was run as negetive 
control on SDS-Page 14% gel. Even with the candy cane marker, 
nonglycosylated protein gives signal. Could any share your 
experineses.
Thans, Siva




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