Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Fri May 22 13:06:49 EST 2009
Am 20.05.2009, 17:59 Uhr, schrieb SIVAKUMAR <usiva from ufl.edu>:
> I have been working on archael protein. Does any one could help me to
> figure out the problem in glycosylation staining with Proq emerald 300.
> I am getting non specific staining that means it stains even
> nonglycosylated protein which was run as negetive control on SDS-Page
> 14% gel. Even with the candy cane marker, nonglycosylated protein gives
> signal. Could any share your experineses.
Have you tried the classical PAS-staining, just as a sanity check? Are you
satining in the gels directly or on blots?
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