Glyco stain

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Fri May 22 13:06:49 EST 2009


Am 20.05.2009, 17:59 Uhr, schrieb SIVAKUMAR <usiva from ufl.edu>:

> Hi
> Greetings
> I have been working on archael protein. Does any one could help me to  
> figure out the problem in glycosylation staining with Proq emerald 300.  
> I am getting non specific staining that means it stains even  
> nonglycosylated protein which was run as negetive control on SDS-Page  
> 14% gel. Even with the candy cane marker, nonglycosylated protein gives  
> signal. Could any share your experineses.

Have you tried the classical PAS-staining, just as a sanity check? Are you  
satining in the gels directly or on blots?


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