expression of a subunit

Azam Rahimpour via methods%40net.bio.net (by rahimpour_a from yahoo.com)
Tue May 26 11:50:39 EST 2009


hi
I am going to express a mutant subunit of a multimeric complex protein in mammalian cells , this subunit is expected to incorporate in the whole protein complex, I dont know if attachment of small tags like his tag or HA tag would disrupt its structure and prevent its incorporation in a complex. does any one has any experience? 


      


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