expression of a subunit
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Fri May 29 12:07:27 EST 2009
Am 26.05.2009, 12:50 Uhr, schrieb Azam Rahimpour <rahimpour_a from yahoo.com>:
> I am going to express a mutant subunit of a multimeric complex protein
> in mammalian cells , this subunit is expected to incorporate in the
> whole protein complex, I dont know if attachment of small tags like his
> tag or HA tag would disrupt its structure and prevent its incorporation
> in a complex. does any one has any experience?
If the 3D structure has been solved (check the PDB), you can look wether
C- or N-terminus is free (solvent exposed). If that is the case, puting a
tag there should not overly affect folding and association. The same is
true for exposed loops, but keep in mind that they may be a binding site
for regulatory proteins (if that is important to you).
If the structure is not known, then you'll have to use trial and error.
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