Restriction Enzyme Help: Sac II

L Celeste via methods%40net.bio.net (by celesteel from gmail.com)
Fri May 29 18:05:48 EST 2009


Hi everyone,

I have been trying to amplify two segments ( 9 kb and 11 kb). I was able to
amplify the right-zed fragment with a primer set containing Sac II. After
restriction enzyme digestion with Sac II, I tried to ligate these two
fragments together. But they fail to ligate but the positve control worked.
Any help would be much appreciated. Thank you!


Celeste


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