Restriction Enzyme Help: Sac II

[L Celeste]

Hi everyone,

I have been trying to amplify two segments ( 9 kb and 11 kb). I was able to
amplify the right-zed fragment with a primer set containing Sac II. After
restriction enzyme digestion with Sac II, I tried to ligate these two
fragments together. But they fail to ligate but the positve control worked.
Any help would be much appreciated. Thank you!


Celeste