no antibiotics when transfecting/infecting. Why?
(by nobody from nospam.not)
Fri May 29 20:25:58 EST 2009
dk from no.email.thankstospam.net (DK) wrote in
news:BP%Tl.81347$uD3.48715 from newsfe20.iad:
> In article <Xns9C1AD75F2DF8Cikkezelf from 22.214.171.124>, Han
> <nobody from nospam.not> wrote:
>>Just showing my ignorance. I just overheard people talk about failing
>>infections (lentivirus/AAV2) or transfections when the cells to be
>>infected or transfected are in antibiotic containing media. Why is
> Details? What antybiotics - the usual anti-bacterial ones or the
> selective drug? No experience with lentivirus but can tell about
> transfections: if the former, then the claims are simply not true -
> transfections work fine with the pen/strep or gentamicin; if the
> latter, then the reason for not working should be obvious.
It was the usual pen/strep. Thanks for the quick reply. I'll have to
needle my colleagues. They stated that it was well-known it didn't work,
but couldn't explain why.
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