no antibiotics when transfecting/infecting. Why?
(by aawara from pontiff-playground.org)
Sat May 30 09:52:11 EST 2009
In <Xns9C1ADA06B9F68ikkezelf from 18.104.22.168>,
Han <nobody from nospam.not> wrote:
>> Details? What antybiotics - the usual anti-bacterial ones or the
>> selective drug? No experience with lentivirus but can tell about
>> transfections: if the former, then the claims are simply not true -
>> transfections work fine with the pen/strep or gentamicin; if the
>> latter, then the reason for not working should be obvious.
> It was the usual pen/strep. Thanks for the quick reply. I'll have to
> needle my colleagues. They stated that it was well-known it didn't work,
> but couldn't explain why.
Agree 100% with Dima. We do lentiviral/retroviral infections all the time,
in media containing antibiotics. Now anti-fungals are another matter. Many
of them impact the infectivity of enveloped viruses that contain cholesterol
rich lipid membranes - like retroviruses and lentiviruses.
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