no antibiotics when transfecting/infecting. Why?

Aawara Chowdhury via (by aawara from
Sat May 30 09:52:11 EST 2009

In <Xns9C1ADA06B9F68ikkezelf from>,
 Han <nobody from nospam.not> wrote:

>> Details? What antybiotics - the usual anti-bacterial ones or the  
>> selective drug? No experience with lentivirus but can tell about 
>> transfections: if the former, then the claims are simply not true -
>> transfections work fine with the pen/strep or gentamicin; if the 
>> latter, then the reason for not working should be obvious. 
>> DK
> It was the usual pen/strep.  Thanks for the quick reply.  I'll have to 
> needle my colleagues.  They stated that it was well-known it didn't work, 
> but couldn't explain why.

Agree 100% with Dima.  We do lentiviral/retroviral infections all the time,
in media containing antibiotics. Now anti-fungals are another matter. Many
of them impact the infectivity of enveloped viruses that contain cholesterol
rich lipid membranes - like retroviruses and lentiviruses.


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