no antibiotics when transfecting/infecting. Why?
(by nobody from nospam.not)
Sat May 30 16:54:23 EST 2009
Aawara Chowdhury <aawara from pontiff-playground.org> wrote in
news:vKbUl.117450$HZ1.10056 from newsfe15.iad:
> In <Xns9C1ADA06B9F68ikkezelf from 188.8.131.52>,
> Han <nobody from nospam.not> wrote:
>>> Details? What antybiotics - the usual anti-bacterial ones or the
>>> selective drug? No experience with lentivirus but can tell about
>>> transfections: if the former, then the claims are simply not true -
>>> transfections work fine with the pen/strep or gentamicin; if the
>>> latter, then the reason for not working should be obvious.
>> It was the usual pen/strep. Thanks for the quick reply. I'll have
>> to needle my colleagues. They stated that it was well-known it
>> didn't work, but couldn't explain why.
> Agree 100% with Dima. We do lentiviral/retroviral infections all the
> time, in media containing antibiotics. Now anti-fungals are another
> matter. Many of them impact the infectivity of enveloped viruses that
> contain cholesterol rich lipid membranes - like retroviruses and
Thanks, Aawara. I can easily see antifungals interfering with membrane
email address is invalid
More information about the Methods