tm calculation for tagged primers

Azam Rahimpour via (by rahimpour_a from
Mon Nov 2 12:32:15 EST 2009


I am new to primer design and I have a simple question: when I add extra sequences like restriction sites and epitope tags to my primer, should I calculate primer tm with these sequences or not? actually when I calculate Tm with these sequences the tm is about 80 and if I ignore them it seems that the calculation isnt complete.

is there any rule for that?



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