(no subject)

Allan Jones via methods%40net.bio.net (by allan.jones from gmx.de)
Tue Nov 3 11:58:41 EST 2009


Hi!

Does anyone here know how stable Adenovirus (not replcation competent) is in mice? I was weighing out pulverized liver tissue of such mice and when cleaning my spatula afterwards with a wet cloth, splashed some of the liquid into my face (incl. lips). My labmates said that there will be very little to no intact virus in the animals after seven days,when the experiment ende (as it is not replication competent and should have either infected cells or been cleared by the mouse's immune system after this time).

Does anyone here know if this isthecase, as i am a little worried about having an AV expressing shRNA in my cells. The doc in the building noted the accident, saying that replication incompetent viruses will not so any harm, especially Adenovirus, as it does not integrate, but i am still a little worried and unsure of how to handle this.

Best Wishes!
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