Mold problem in 4°C room

Dr. Hiranya S. Roychowdhury via (by hroychow from
Thu Nov 5 14:54:12 EST 2009

We have used 8-10% bleach successfully on non-porous surfaces.  Stainless
steel benches and tables only should be allowed in a cold rooms.  We have
had to discard any wooden drawers or boxes (and of course those infernal
cardboard boxes) from the cold rooms.

We wiped the surfaces down and left the bleach solution over the weekend.

> Hello everyone,
> we have a problem with mold and its spores in our 4°C room. It was used as
> a storage room for several kinds of mold (in unwrapped/unsealed petri
> dishes) in the last few years while the former department was studying it.
> Now, this research has been discontinued and we are trying to use it as a
> "standard" cold room for storage of molecular biology reagents, protein
> purification purposes and so on. However, you can still smell the mold,
> and LB agar plates show signs of mold shortly after they have been put
> there.
> Does anybody know how to decontaminate it thoroughly? Or at least an idea
> how to do it?
> Thank you very much in advance!
> Greetings,
> Ray
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Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003

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