RNA ligation

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Wed Nov 18 20:54:25 EST 2009


On Nov 18, 11:35 am, Chris McDermott-Roe
<cjmcdermott... from googlemail.com> wrote:
> Hi everyone,
>
> I hoping to ligate a synthesised oligonucleotide to the 5' end (uncapped but
> not dephosphorylated) of an mRNA population.  Can anybody tell me the best
> way of doing this?
>
> many thanks
>
> Chris

Are they both single stranded? I think T4 RNA ligase will do that
reaction.

See:
<http://www.neb.com/nebecomm/products/productM0204.asp>

Nick

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Nick Theodorakis
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