RNA ligation

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Fri Nov 20 21:58:13 EST 2009


In <4e7e0a8e-85b3-4eda-9190-7740ce3fc37e from p35g2000yqh.googlegroups.com>,
 Nick Theodorakis <nick.theodorakis from gmail.com> wrote:

>>
>> Won't work for mRNA - there is no free 5'phosphate.
>>
>
> He specified that his RNA population is uncapped and phosphorylated in
> his original post. I don't know how it got that way, but I can imagine
> at least one scenario.

Then it cannot be mRNA.  It could be pre-mRNA or hnRNA, but mRNA needs
to be capped.

AC
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