Sequential restriction digest

Nikola Wenta via methods%40net.bio.net (by Nikola.Wenta from nottingham.ac.uk)
Sun Nov 22 13:53:25 EST 2009


Dear all!
Of course, using isoschizomers or hifi enzymes are an interesting idea,
but are either time consuming or expensive for only one application. I
have therefore tried the low-salt-buffer-first suggestion, and it really
worked great! Complete digest, no waste of agarose and extraction kits,
simply two-step-single-tube ;-)
Thank you all very much for your suggestions!
   Niko

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