lyophilizing leaf tissue

Jeremy Coate via methods%40net.bio.net (by coatej from gmail.com)
Tue Nov 24 08:46:40 EST 2009


Hi savy methodologists,

I need to lyophilize leaf tissue prior to shipping overseas. The tissue wil=
l
be used for analysis of lipids, which are vulnerable to endogenous lipases,
so I am planning to collect the tissue into liquid N2, freeze dry and ship
on dry ice to try to ensure that these lipases don't have a chance to do
their thing. I have never used a lyophilizer and have concerns that I'm
using it incorrectly, and that the samples are thawing.

I'm using a Labconco FreeZone 18 freeze dryer with a bulk dryer unit on top=


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