Purifying 5' phosphorylated primers for mutagenesis
(by haleyl from u.washington.edu)
Tue Nov 24 17:12:50 EST 2009
I'm doing some site-directed mutagenesis with a kit from NEB. It is
recommended in the kit directions that the 5' phosphorylated primers used
for the reaction are purified either using RP-HPLC or PAGE.
My issue is that these purification methods are *really* expensive. (I've
been getting my primers through Invitrogen, but I've also looked at a few
other places and it's the same story.) Has anyone had success using primers
purified another (less expensive) way or done the purification in house?
Haley, University of Washington
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