Purifying 5' phosphorylated primers for mutagenesis

Ho Leung Ng via methods%40net.bio.net (by holeung.ng from gmail.com)
Fri Nov 27 01:28:16 EST 2009


For most cases, just desalted primers work most of the time. Using
purified primers can help if you use very long primers (50+ nt) or are
doing long insertions/deletions. For problematic primers, I like to
buy from Bioneer, which offers reverse phase purification for free.


ho



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