problem with competent cells

ms via methods%40net.bio.net (by monikagicts1 from gmail.com)
Sat Oct 3 06:29:03 EST 2009


hi all,

i want to share one problem regarding DH5 alpha ultra com cells
preparation using inoue method. i am getting contamination as i am not
sure whether it is contamination exactly i donot know but i am getting
good efficiency. everything i am doing in sterile environment. all
buffers i prepared in hood and filtered through .22 micron filter but
i donot know what is the problem. so,  please suggest me what is the
problem.

ms



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