Mitochondrial isolation for mt DNA extraction from Earthworms

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Wed Oct 7 08:08:14 EST 2009


Am 05.10.2009, 02:48 Uhr, schrieb luiscunhamx <luisnevescunha from gmail.com>:

> Dear collegues
> I wonder if anyone ever tried to isolate mitochondrias from earthworms
> for posterior mtDNA extraction, could anyone send me a good protocol?
> I am having a lot of trouble doing that, probably due to soil
> contamination in the digestive tract, even after depuration and I am
> getting very low yield of mtDNA. Any help?? I am working with
> Pontoscolex corethrurus, my model organism :).

For histology, it is customary to feed earthworms for two weeks on humid  
filter paper pieces, which are changed daily. That removes any soil from  
their digestive tract, which would otherwise nick the microtome knife.


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