3' end labelling with tritiated thymidine...

Rory O'Brien via methods%40net.bio.net (by rory.obrien from stonebow.otago.ac.nz)
Thu Oct 8 23:01:20 EST 2009


Hi Group,

 

I'm looking to try end-tailing an oligonucleotide with terminal transferase
and labeling it with tritium in the process; the tritiated thymidine
products available from Perkin Elmer come as -

 

NET221H - Deoxythymidine 5`-Triphosphate, [Methyl-3H]-, Tetrasodium Salt,
Specific Activity: 10-25Ci (370-925GBq)/mMole, >97%, 1mCi/mL. Packed in
ethanol :water (1:1)

 

NET221X -  Deoxythymidine 5`-Triphosphate, [Methyl-3H]-, Tetrasodium Salt,
Specific Activity: 70-90Ci (2.59-3.33TBq)/mMole, 1mCi/mL.  Packed in ethanol
:water (1:1)

 

NET221A - Deoxythymidine 5`-Triphosphate, [Methyl-3H]-, Tetrasodium Salt,
Specific Activity: 70-90Ci (2.59-3.33TBq)/mMole, 2.5mCi/mL. Packed in 10mM
tricine buffer (pH 7.6)

 

 

Could anyone maybe suggest which thymidine product might be most appropriate
to this application? Detection to be via scintillation counter; one for the
old-school molecular biologists maybe ;-)

 

Cheers,

 

- Rory

 



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