PhiX174 resistance E. coli strain?

Yoram Gerchman via methods%40net.bio.net (by gerchman from research.haifa.ac.il)
Mon Oct 12 12:44:31 EST 2009


Thanks Dunken. Could you kindly elaborate on the EcoB/EcoK issue? Since the
lysis system (E protein) seem to work in K12/coli B whay would not the phage
propagate in them?
Many thanks
Yoram

> Date: Mon, 12 Oct 2009 15:24:57 +0100
> From: Duncan Clark <blackhole from abuse.plus.com>
> Subject: Re: PhiX174 resistance E. coli strain?
>
> Historians believe that in newspost
> <mailman.62.1255029029.1133.methods from net.bio.net> on Thu, 8 Oct 2009,
> Yoram Gerchman <gerchman from research.haifa.ac.il> penned the following
> literary masterpiece:
> >I am looking for PhiX174 resistance E. coli strain. Does anyone know of such
> >strain?
>
> The original hosts for phiX174 production were E.coli C derivatives. No
> idea if it goes into E.coli B or K12 strains. If it's genome has an EcoB
> or EcoK site in the sequence then that would answer the question.
>
> I think a Pubmed search is the best way to go.
>
> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.


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