anti Tag antibody cross reactivity

Azam Rahimpour via methods%40net.bio.net (by rahimpour_a from yahoo.com)
Sun Oct 25 09:16:01 EST 2009


Hi
does anybody use different epitope tags for proteins expressed in mammalian cells? I have some questions about this:
 
1. Is it important to add small tags to either C or N terminal of proteins?
 
2. Is it necessary to put an spacer or specific sequence between tag and protein coding region?
 
3. which anti tag (FLAG, His, myc, HA ...) has lower cross reactivity to cellular proteins?
 
thanks in advance


      


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