Loading dye

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Mon Oct 26 13:05:08 EST 2009


Bromo phenol blue.. Frequently added with xylene cyanol in DNA loading dyes.  

Jay

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Barbara MacGregor
Sent: Monday, October 26, 2009 1:13 PM
To: methods from oat.bio.indiana.edu
Subject: Re: Loading dye

Xylene cyanol definitely has a different color, though, at least as I've used it - blue-green rather than blue.

But those are the only blue dyes I can think of, so that's probably it...

Barbara

>
> Raymond Dalgleish <rwmd1 from NOSPAMle.ac.uk> wrote:
>
>> Any ideas about what the blue dye might have been in this incorrect 
>> loading dye?
>
> This sounds very much like Xylene cyanol. Our loading biuffer contains 
> both dyes.
>
> Christian
>

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