David-Paul Minde via (by davidminde from
Mon Oct 26 21:06:44 EST 2009

how about DMSO ? if available, I think the additive of choice (because about
everybody uses it and therefore tons of experience reports on it around).

2009/10/27 Bari Zahedi <bzahedi from>

> Has anyone used PEG to reduce melting temperature of their primers for PCR?
> We are working with very GC rich DNA and have been using Betane but we have
> read that MEG works better. We don't have any MEG but we do have PEG and
> were wondering if it could be substituted for MEG. Thanks in advance.
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David Minde MSc (TUM)

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