MEG vs. PEG
(by davidminde from gmail.com)
Mon Oct 26 21:06:44 EST 2009
how about DMSO ? if available, I think the additive of choice (because about
everybody uses it and therefore tons of experience reports on it around).
2009/10/27 Bari Zahedi <bzahedi from bccrc.ca>
> Has anyone used PEG to reduce melting temperature of their primers for PCR?
> We are working with very GC rich DNA and have been using Betane but we have
> read that MEG works better. We don't have any MEG but we do have PEG and
> were wondering if it could be substituted for MEG. Thanks in advance.
> Methods mailing list
> Methods from net.bio.net
David Minde MSc (TUM)
Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
NL-3584 CH Utrecht
office phone +31 30 253 4105
(magic:) android +31 652614443
Griftkade 4 bis
3572 TW Utrecht
Science is what happens while we are making other plans (~John Lennon)
More information about the Methods